Effects of Methyl Jasmonate on Rotenone-Induced Microglial Activation in Parkinsonian Mice
Type
Thesis
Authors
Category
Physiology
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Publication Year
2023
Subject
A research project in physiology
Abstract
Parkinson's disease is an extrapyramidal motor disorder marked by rigidity and tremor. It
stems from oxidative stress and the degeneration of dopaminergic neurons, particularly
affecting the nigrostriatal pathway. This has led to intensified research into compounds
capable of mitigating oxidative stress, neurodegeneration and neuroinflammation, as they
offer promise as therapeutic options for managing Parkinson's disease. The study investigated
the anti-oxidative and neuroprotective effects of Methyl Jasmonate (MJ) level in rotenoneinduced
microglial activation in parkinsonian mice. 50 male Swiss mice were administered
with MJ (100 mg/kg) daily following alternate rotenone (2.5 mg/kg) treatment via
intraperitoneal injection. The impact of MJ on motor impairments induced by rotenone were
assessed while endogenous antioxidant status (Malondialdehyde (MDA) nitrite; glutathione
(GSH)), dopamine level and ionized calcium binding adapter molecule-1 (IBA-1) were
evaluated on the striatum using spectrophotometric and ELISA method. The result showed
that the mice exposed to rotenone treatment displayed a significant (p < 0.05) reduction in
sensorimotor and neuromuscular performance as indicated by a significant (p < 0.05)
increase in ambulation and muscle movement and a significant (p < 0.05) decrease in
akinesia/catalepsy. The striatal rotenone lesioning significantly (p < 0.05) heightened the
levels of MDA but significantly (p < 0.05) decrease the release of endogenous GSH in the
striatum. However, treatment with MJ significantly (p < 0.05) decreased the MDA as well as
significantly (p < 0.05) increased the release of endogenous GSH in the striatal tissue.
Microglial protein expression (IBA 1) was observed to be remarkably (p < 0.05) activated
with concomitant significant (p < 0.05) dopamine reduction in the striatal rotenone lesioned
mice which was further attenuated following treatment with MJ. The results of this study
indicated that MJ exhibits qualities akin to anti-parkinsonic effects, potentially attributed to its
capacity to hinder oxidative stress, mitigate the deterioration of dopaminergic neurons, and
suppress the expression of microglia cell activation.
stems from oxidative stress and the degeneration of dopaminergic neurons, particularly
affecting the nigrostriatal pathway. This has led to intensified research into compounds
capable of mitigating oxidative stress, neurodegeneration and neuroinflammation, as they
offer promise as therapeutic options for managing Parkinson's disease. The study investigated
the anti-oxidative and neuroprotective effects of Methyl Jasmonate (MJ) level in rotenoneinduced
microglial activation in parkinsonian mice. 50 male Swiss mice were administered
with MJ (100 mg/kg) daily following alternate rotenone (2.5 mg/kg) treatment via
intraperitoneal injection. The impact of MJ on motor impairments induced by rotenone were
assessed while endogenous antioxidant status (Malondialdehyde (MDA) nitrite; glutathione
(GSH)), dopamine level and ionized calcium binding adapter molecule-1 (IBA-1) were
evaluated on the striatum using spectrophotometric and ELISA method. The result showed
that the mice exposed to rotenone treatment displayed a significant (p < 0.05) reduction in
sensorimotor and neuromuscular performance as indicated by a significant (p < 0.05)
increase in ambulation and muscle movement and a significant (p < 0.05) decrease in
akinesia/catalepsy. The striatal rotenone lesioning significantly (p < 0.05) heightened the
levels of MDA but significantly (p < 0.05) decrease the release of endogenous GSH in the
striatum. However, treatment with MJ significantly (p < 0.05) decreased the MDA as well as
significantly (p < 0.05) increased the release of endogenous GSH in the striatal tissue.
Microglial protein expression (IBA 1) was observed to be remarkably (p < 0.05) activated
with concomitant significant (p < 0.05) dopamine reduction in the striatal rotenone lesioned
mice which was further attenuated following treatment with MJ. The results of this study
indicated that MJ exhibits qualities akin to anti-parkinsonic effects, potentially attributed to its
capacity to hinder oxidative stress, mitigate the deterioration of dopaminergic neurons, and
suppress the expression of microglia cell activation.
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